colocalize.com © 2008

No More Noise

Advantages using RBNCC:

You get the correct answer.

You can make measurements from very poor images.

Your imaging time can be shortened and photobleaching therefore reduced.

Shorter imaging times means that the cells move less during image acquisition.

You can get more images out of your fluorophores making longer time series possible.

Phototoxicity is reduced which is important for live cell imaging.

A detailed description of RBNCC can be found at:

Replicate Based Noise Corrected Correlation for Accurate Measurements of Colocalization. Journal of Microscopy 230, 121-133 (2008) Adler, J., Pagakis, S. & Parmryd, I.

Mathematical proof of why and how RBNCC works can be found in:

Analysis of bias in the apparent correlation coefficient between image pairs corrupted by severe noise. Journal of Mathematical Imaging and Vision 37, 204-219 (2010) Bergholm, F., Adler, J. & Parmryd, I. Journal of Mathematical Imaging and Vision

In a review article we describe RBNCC in more general terms:

Noise and colocalisation in fluorescence microscopy: solving a problem. Microscopy and Analysis 22, 7-10 (2008) Adler, J., Bergholm, F., Pagakis, S. & Parmryd, I. Microscopy and Analysis

Step-by-step protocols for quantitative analysis of colocalization divided into co-occurrence and correlation analyses including RBNCC can be found in:

Colocalization Analysis in Fluorescence Microscopy (2012) Adler, J. & Parmryd, I. Methods in Molecular Biology

A detailed demonstration of why only pixels containing both fluorophores should be included in a correlation analysis.

Quantifying colocalization: thesholding, void voxels and the Hcoef. PLoS One 9:e111983 Adler, J. & Parmryd, I. (2014) PLoS One

NEWS

November 2014. Article demonstrating why only pixels containing both fluorophores should be used for correlation alalysis. Aritcle.

September 2013. Patent granted in Japan.

June 2012. Book chapter describing the use of RBNCC in a step-by-step protocol Chapter.

March 2012. Six month trial licence of RBNCC available from SVI.

February 2012. Patent granted in the United States.

January 2011. No More Noise signs agreement with Scientific Volume Imaging to use RBNCC.

April 2010. Article comparing colocalisation coefficients.

March 2010. Article proving that RBNCC is sound from a mathematical perspective.

November 2008. The people behind RBNCC were awarded Stockholm's Inventors Prize. Press release.

September 2008. Review on RBNCC in Microscopy and Analysis.

July 2008. Note on RBNCC in Microcopy and Analysis.

April 2008. Article describing RBNCC in Journal of Microscopy.